The Effect of Microwave Emission from Mobile Phones on Neuron Survival in Rat Central Nervous System

To investigate the effect of microwave emitted by mobile phones on the rat central nervous system (CNS), in vitro cultured cortical neuronal cells and in vivo rat’s brain were exposed to the electromagnetic waves emitted by a microwave transmitter that mimics the working frequency of mobile phones. Trypan blue staining and terminal deoxynucleotidy transferase-mediated dUTP nick-end labeling (TUNEL) were used to determine the survival state of neuronal cells while immunohistochemistry method was used to determine the expression level of Bcl-2 and Bax. Our results show that microwave lead to significant cell death in culture and more in vivo brain neuronal cells were stained positive for TUNEL, Bax and Bcl-2 in rats with cranial defect after exposure than that for † To whom correspondence should be addressed: Department of Neurosurgery, the Second Affiliated Hospital of Zhejiang University School of Medicine, 88 Jiefang Road, Hangzhou 310009, Zhejiang Province, People’s Republic of China